Synthesis of curcumin β-maltooligosaccharides through biocatalytic glycosylation with Strophanthus gratus cell culture and cyclodextrin glucanotransferase

Abstract

A two step synthesis of a series of curcumin β-maltooligosaccharides through sequential biocatalytic glycosylation using Strophanthus gratus cell culture and cyclodextrin glucanotransferase (CGTase) is reported. Cultured plant cells of S. gratus converted exogenously added curcumin into unnatural curcumin β- d-glucoside. Furthermore, four unnatural β-maltooligosaccharides, that is, α-Glc-1→(4-α-Glc-1→) n−1 4-β- d-glucosides ( n = 1–4), of curcumin were produced from curcumin β- d-glucoside in the presence of starch by CGTase-catalyzed glycosylation.