Abstract
Chlamydomonas reinhardtii cells provide an effective system for glycolate photoproduction, operative during 30 h when they are growing under low CO2, in the presence of 1 mM aminooxyacetate and 50 μM acetazolamide. Glycolate excretion by the cells can proceed for about 4 days if every other 12 h a high CO2 level is restored in the culture in the absence of inhibitors. The immobilized system in alginate beads has about a twofold higher glycolate photoproduction rate (23 μmol·mg chlorophyll (Chl)−1·h−1) than free-living cells (12 μmol · mg Chl−1 · h−1).
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