Abstract
1406 This study was conducted to determine endogenous glucose oxidation during prolonged running following a carbohydrate (CHO) diet labeled with 13C. Six male runners (VO2max, 60.0±2.2 ml·kg-1·min-1) performed two 120-min treadmill exercises (65% maximal speed; 69±2 VO2max). A first exercise was performed following an overnight fast and a light breakfast, and was followed by a cycling bout of exercise to exhaustion (Trial A). Then, over the next 48 hrs, subjects ingested a high (CHO) diet (1250 g, 90% CHO) artifically labeled with 13C-glucose and then performed a second exercise (Trial B). The average VO2, energy cost, and protein oxidation were significantly(p<0.05) lower in trial B than A (2.85±0.10 vs. 2.93±0.10 L·min-1; 14.38±0.38 vs. 14.72±0.50 kcal·min-1 and 0.07±0.001 vs. 0.11±0.008 g·min-1 respectively). Results from indirect calorimetry (IC), show no differences for fat and total CHO oxidations between trials (fat, 0.52±0.08 vs. 0.49±0.07; CHO, 2.34±0.23 vs. 2.38±0.24 g·min-1 in trials A and B respectively). Calculations of CHO oxidation from expired 13VCO2 was similar to that derived from IC: 1st hr, 1.19±0.10 vs 1.26±0.10 g·min-1; 2nd hr, 1.03±0.21 vs 1.12±0.13 g·min-1 for 13VCO2 and IC respectively. These results indicate that hepatic and muscle glycogen reserves can be adequately labeled with 13C-CHO with an exercise-diet regimen.
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