Glycogen; its ultrastructural staining characteristics and distribution in some nematodes.

Abstract

In view of erratic staining of glycogen in thin sections of nematode tissues, a study of cytochemical staining using the periodic acid-thiosemicarbazidesilver protein technique along with diastase control reactions was carried out on the nematode Capillaria hepatica. Glycogen is readily demonstrated by this technique and its morphology and distribution has been examined in six nematode species. This cytochemical technique is recommended for accurate assessment of the glycogen content of various cell types in the hope that accurate correlations between metabolic activity and polysaccharide reserves may be drawn.