Abstract
Using quantitative laser-scanning microtomography (QLSM), the response of mature mouse oocytes to hypotonic stress was investigated. The response exhibited the following features: (1) oocytes were capable of regulatory volume decrease (RVD); (2) unlike zygote and two-cell embryo, the oocyte volume recovery was not complete even by 120 min; (3) millimolar and submillimolar concentrations of glycine arrested the development of RVD in oocytes.
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