Abstract
Summary In pumpkin plants ( Cucurbita pepo L.) grown in open-top chambers under polluted air, the levels of metabolites and related enzymatic activities involved in the glutathione-ascorbic acid cycle were analyzed. The plants grown in ambient and unfiltered air exhibited an unchanged hydrogen peroxide level but an increased membrane lipid peroxidation index (measured as malondialdehyde content). The content of reduced forms of glutathione and ascorbic acid increased in the polluted plants together with the enzymatic activities of glutathione reductase and dehydroascorbate reductase involved in their recycling. The ascorbate peroxidase activity remained unchanged in all samples. The data obtained support the hypothesis that in pumpkin plants, the glutathione-ascorbic acid cycle is stimulated in removing the hydrogen peroxide produced under oxidative stress.
Published Version
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