Glutathione S- Transferase M1 in the Serum of Patients with Endometriosis

Zahra Sorkhoo

doi:10.5539/jmbr.v6n1p67

Abstract

<p class="1Body">Endometriosis is a female disease with high incidence and unknown causes because of the presence of endometrial tissue outside of the uterine wall. Glutathione S- transferase (GSTs) is a key enzyme in phase I and II that catalyzes the conjugation of glutathione to genotoxic compounds. Glutathione S- transferase M1 (GSTM1) is a versatile enzyme that plays an important role in detoxification of carcinogenic metabolites. The aim of this study was to evaluate serum levels GSTM1 in patients with endometriosis. In this study, blood samples from 20 women with endometriosis and 20 healthy women were collected. The final concentration of protein in the blood of patients (TPC) and GSTM1 in serum by using "biuret" and enzyme-linked immunosorbent assay "(ELISA) were measured. It was shown that the level of total protein concentration all samples are within the normal range and no significant changes between the two groups were seen. Although significant reduction in serum levels GSTM1 was observed in patients with Endometriosis compared to the control group (P = 0.002). The results of this study showed that GSTM1 is involved pathophysiology of endometriosis.</p>

Highlights

Endometriosis is the presence of endometrial tissue outside the uterus is believed to play an important role in infertility (Thornton, Morley, Lilleyman & Onwude, 1997)
The results show GSTP1 polymorphism associated with the risk of endometriosis risk (Ertunc et al, 2005)
The ELISA technique to study protein level Glutathione S- transferase M1 (GSTM1) in patients with endometriosis compared with healthy subjects showed that the average concentration in the blood serum of women with endometriosis GSTM1 mu / ml 1.35 ± 4.16, compared with the average concentration of serum GSTM1 healthy female blood mu / ml 1.79 ± 5.82 decreased significantly show that at P=0.002 (Figure 2)

Summary

Introduction

Endometriosis is the presence of endometrial tissue outside the uterus is believed to play an important role in infertility (Thornton, Morley, Lilleyman & Onwude, 1997). Glutathione-S-transferase M1 gene is set on chromosome 1p13 and expresses the enzymes GSTM1 (Zhong, Wolf & Spurr, 1992). GSTM1 gene deletion on the impact and cause defects in the detoxification enzyme and the risk of exposure to carcinogens and toxic chemicals will increase (Seidegard, Vorachek, Pero & Person, 1988). GSTM1 gene deletion in diseases such as ovarian cancer (Baxter, Thomas & Campbell, 2001), Cystic fibrosis (Baranova et al, 1997), gastric cancer Harada et al, 1992), lung cancer (Nakachi et al, 1993) and bladder cancer (Brockmoller et al, 1994) have been reported. Studies have shown that GSTM1 gene is removed in a high percentage of women with endometriosis (Nisolle et al, 1990)

Objectives

Methods

Results