Abstract
Many different biomass of agricultural origin holds remarkable potential for conversion into valuable products thereby presenting a double sharp edge importance of sustainable resource supply and environmental protection. Glutamic acid was produced from rice husk using a novel strain of Corynebacterium glutamicum and effects of parameters optimization such as substrate concentration, temperature, pH and inoculum size were determined during the fermentation process. The wild-type (Novel) strain was inoculated into 13 g/L of the pre-treated rice husk previously added to basal medium (pH 7.2), after which fermentation began. Fermentation broth from each flask was taken aseptically after 96 h and was assayed qualitatively and quantitatively. The acid-treated and alkali-treated rice husk gave the best glutamic acid yield of 10.40g/L and 9.08g/L respectively with the wild-type strain under predetermined optimum fermentation conditions. Out of the four parameters optimized, only substrate concentration was not found to be significant on the performance of the wild-type strain in glutamate production (p > 0.05). Acid-treated rice husk hydrolysate was found to be a better substrate for L-glutamate production by the wild-type strain of C. glutamicum under the optimum fermentation conditions determined.
Highlights
One of the most importantnon-essential amino acids is LGlutamate
The isolate from chicken-pen named as SFCD2 gave the maximum (0.25g/L) L-glutamic acid yield, with isolate from sheep-pen named as SFSD1 giving the least yield of 0.12g/L when determined spectrophotometrically at 570nm with reference to standard curve
Various concentrations (1 to 5%) of each of the pre-treated substrates were investigated and it was found that 4% (w/v) of the acid-treated rice husk gave the highest production of glutamic acid (6.37 g/L) after 96 hours of incubation
Summary
One of the most importantnon-essential amino acids is LGlutamate. It is widely used in foods as a flavour enhancer. Corynebacterium glutamicum is catalase-producing and break down carbohydrates by fermentative metabolism (Blombach and Seibold, 2010). This organism is auxotrophic to biotin but secretes L-glutamic acid in response to biotin limitation (Nottebrock et al, 2003). Several different substrates such as glucose, beet molasses and cassava residues are used for the production of glutamic acid. Optimum glutamic acid yield is obtained under optimum fermentation parameters irrespective of the strain type; whether wild or mutant forms of any organism capable of producing this particular amino acid
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