Abstract
Ceiba pentandra (L.) stem bark plant contains tannin, flavonoid, alkaloid, terpenoid, and saponin compounds. It is known that flavonoid compounds have a potential for inhibition activity of an α-glucosidase enzyme. The purpose of this research is to know the α-glucosidase inhibition activity of the fraction of n-hexane, ethyl acetate, and water. These three fractions measured α-glucosidase inhibition activity by spectrophotometric UV-vis method. The fraction extract with IC 50 values can best be done an analysis of active compounds using LCMS/MS methods, and results of the suspected compound do the binding molecule with a receptor model isomaltase α-glucosidase uses AutoDock Vina so that bond can predict drug active compounds. The results of this research activity are inhibition of α-glucosidase stem bark in the fraction of n-hexane 4,60 μg/mL, ethyl acetate fraction 8,55 μg/mL and fractions of the water 5,61 μg/mL. Analysis of the compound fraction of water by LCMS/MS eluted at the retention time 3,61 seconds allegedly derivative compounds (+) catechin and on retention time 8,70 minutes allegedly derivative compounds vavain. Vavain derivative compounds, (+) catechin, acarbose and quercetin each has its own docking score of -8,1; -8,8; -6,2 and -7,6 kcal/mol and has a semblance of bonding on amino acid Glu276 which is a residue catalytic role in hydrolysis reactions. Equality on the sides of active chemical bonds is expected to have the same activity as drug inhibition of the enzyme α-glucosidase.
Highlights
Diabetes mellitus (DM) is a chronic disease occurs either when the pancreas does not produce enough insulin or when the body cannot effectively use the insulin produced
World Health Organization (WHO) states that there are 422 million adults living with diabetes in 2014 in the world
The prevalence of people with diabetes in Indonesia has been increased from 5.7% in 2007 to 6,9% in 2013 and it becomes highest cause of death number 3 in Indonesia (World Health Organization, 2016)
Summary
Diabetes mellitus (DM) is a chronic disease occurs either when the pancreas does not produce enough insulin or when the body cannot effectively use the insulin produced. World Health Organization (WHO) states that there are 422 million adults living with diabetes in 2014 in the world. The prevalence of people with diabetes in Indonesia has been increased from 5.7% in 2007 to 6,9% in 2013 and it becomes highest cause of death number 3 in Indonesia (World Health Organization, 2016). Pharmacological therapy can be proceed with oral antidiabetic medication that is one of them α-glucosidase inhibitor acarbose, miglitol, voglibose, and deoxynojirimycin. These drugs work as competitive inhibitor of enzymatic breakage of maltase, isomaltase, sucrose and glyucoamylase inhibitor to delay the decomposition of sucrose and complex of carbohydrate in the intestine. The main effect is reducing glucose level in blood. (Wells et al, 2009)
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