Abstract
Cytotoxic test of fig leaf ethanol extract fraction (Ficus carica L.) has been conducted with the Brine Shrimp Lethality Test (BSLT) method. This study aims to determine the cytotoxic activity of fig leaf ethanol extract using the Brine Shrimp Lethality Test (BSLT) method. Fig leaves are extracted by maceration using 70% ethanol, then proceed with fractionation based on polarity, fractionation using N-hexane solvent, ethyl acetate and water. The results of phytochemical screening of fig leaf ethanol extract fraction stated that, n-hexan fraction contains flavonoid, phenolic, triterpenoid and saponin compounds. The ethyl acetate fraction contains phenolic compounds, triterpenoids and saponins. While the water fraction contains flavonoids, phenolics and saponin compounds. The results of cytotoxic testing of the fraction of fig leaf ethanol extract obtained LC50 values in the n-hexane fraction were 41.78 ppm (toxic), ethyl acetate fraction 33.57 ppm (toxic), and water fraction 16.36 ppm (very toxic).
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