Abstract
This study measures in situ microbial glucose uptake rate in two different hot springs in Ladakh, J&K, India with distinct temperatures >74°C and pH > 7.4. For this purpose, the slurry samples from each hot spring were incubated up to 4 h with 13 C-labelled glucose in gas-tight incubation bottles at the respective hot-spring sites. The natural δ 13 C particulate varies from –9.1‰ in Panamik hot spring to –11.7‰ in Puga hot spring. After incubation with 13 C-labelled glucose, the δ 13 C particulate reached a maximum 2472‰ in Panamik and 4365‰ in Puga hot-spring samples. The glucose uptake rate calculated from the final δ 13 C particulate in the incubation bottles varied from 28 to 147 ng C g –1 h –1 in the Panamik and from 168 to 1196 ng C g –1 h –1 in the Puga samples. This reveals that even at >74°C temperature, thermophiles are capable of running their metabolic machinery, perhaps faster than the heterotrophic microbes/cells under normal temperature condition.
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