Glucose-Sensitive Polypyrrole/Poly(Styrenesulfonate) Films Containing Co-immobilized Glucose Oxidase and (Ferrocenylmethyl) Trimethylammonium Bromide

Abstract

Abstract Glucose oxidase (GOx) and (ferrocenylmethyl) trimethylammonium bromide (FcBr) were co-immobilized into polypyrrole (PP)/poly(styrenesulfonate) (PSS) films during the electropolymerization of pyrrole. PSS was employed as a partial dopant anion for PP (along with Br−) and as an electrostatic binder for the cationic mediator (FcBr). The electropolymerization of the PP/PSS film was carried out at +0.8 V vs SCE in a 5-cm3 aqueous solution of glucose oxidase (1 mg°Cm−3), FcBr (1 mM), PSS (1 mM), and pyrrole (50 mM) at 5°C. These enzymemediator derivatized electroactive polymer films (ca. 0.5-2 μm thick) coated on Pt electrodes showed sensitivities to glucose without the use of any mediator in solution, and can be used as amperometric glucose sensors. The responses of the resulting electrodes (0.24 cm2 Pt plate with the polymer film) to glucose were measured amperometrically in pH 7, 0.1 M phosphate buffer solution at 5°C, and a constant potential (+0.5 V vs SCE) by using a Pt coil counterelectrode, wit...