Abstract
Au nanoparticles (NPs) have been found to be excellent glucose oxidase mimics, while the catalytic processes have rarely been studied. Here, we reveal that the process of glucose oxidation catalyzed by Au NPs is as the same as that of natural glucose oxidase, namely, a two-step reaction including the dehydrogenation of glucose and the subsequent reduction of O2 to H2O2 by two electrons. Pt, Pd, Ru, Rh, and Ir NPs can also catalyze the dehydrogenation of glucose, except that O2 is preferably reduced to H2O. By the electron transfer feature of noble metal NPs, we overcame the limitation that H2O2 must be produced in the traditional two-step glucose assay and realize the rapid colorimetric detections of glucose. Inspired by the electron transport pathway in the catalytic process of natural enzymes, noble metal NPs have also been found to mimic various enzymatic electron transfer reactions including cytochrome c, coenzymes as well as nitrobenzene reductions.
Highlights
Au nanoparticles (NPs) have been found to be excellent glucose oxidase mimics, while the catalytic processes have rarely been studied
Au NPs are structurally different from the active site of glucose oxidase (GOD) but show GOD-like activity, which is highly effective for the oxidation of glucose to produce H2O2 and has attracted substantial interest in biological detection and therapeutics[6,7]
The generated H2O2 was detected by horseradish peroxidase (HRP)based colorimetric system using 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromic indicator
Summary
Au nanoparticles (NPs) have been found to be excellent glucose oxidase mimics, while the catalytic processes have rarely been studied. These results indicate that Au NPs catalyzes the transfer of electrons from glucose to O2 and the reduction of O2 to H2O2. The gathered results in our study draw a possible GOD-like mechanism for noble metal nanozyme-catalyzed glucose oxidation.
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