Abstract
AbstractThe production of extracellular glucose oxidase in a submerged culture by a number of auxotrophic, 2‐deoxy‐D‐glucose resistant and protease‐less mutants of Aspergillus niger was evaluated. Among the auxotrophic strains, no evident dependence was found between the kind of the nutritional requirements and the level of the glucose oxidase activity. However, the majority of auxotrophs, requiring serine or niacin, showed a higher enzyme activity (from 16 to 680%) than the parent strain. The dynamics of the glucose oxidase synthesis by the free and immobilized mycelium of the most active niacin− mutant of A. niger was also investigated.
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