Abstract
The present study is concerned with the production of glucose oxidase (GOD) from thermophilic Penicillium sp . in 250 mL shake flask. Fourteen different strains of thermophilic Penicillium sp. were isolated from the soil and were screened for glucose oxidase production. IIBP-13 strain gave maximum extra-cellular glucose oxidase production as compared to other isolates. Effect of submerged fermentation in shaking and static conditions, different carbon sources and incubation period on the production of extra-cellular glucose oxidase was studied. Maximum yield (0.325 U/mL) of extra-cellular glucose oxidase was obtained after 72 hrs of incubation at 45 o C using submerged fermentation technique in shaking conditions utilizing sucrose as carbon source.
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