Abstract
Dietary fiber [DF] has received considerable attention because of its possible beneficial effects for human health; thus, several methods have been attempted for analysis and characterization of DF. In this paper the valuation of the fiber composition on some cereal products is reported. The fiber determination has been carried out by means of three procedures, different for derivatization conditions and chromatographic separation techniques; the first two, yet described in previous papers, are based on derivatization of monoses to alditol acetates or nitrile-peracetates and on GLC or HRGC separation respectively. The third procedure regards a new attempt, based on derivarization of alditols [obtained from monoses] by phenylisocianate to corresponding urethanes and on their separation by gradient RP-HPLC. The results show that the RP-HPLC procedure can be considered a valid alternative to GC techniques.
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