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Abstract
Methods and results of culturing human blood and bone marrow cells on glass substrates are reported. A pattern of culture divisible into three successive but overlapping phases was observed. Cells of the first phase, recognisable as normal blood cells, are more suitably studied by other techniques. The large granular cells of the second phase and the fibroblast-like cells of the third phase are described and their origin discussed. Glass substrate cultures of bone marrow cells are a convenient and reliable source of human fibroblast-like cells for morphological, physiological and pharmocological experiments. They have no value as an aid to diagnosis.
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