Abstract
We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the binding of MNPs to the GMR sensor and the binding is proportional to the concentration of virus. Binding of MNPs onto the GMR sensor causes change in the resistance of sensor, which is measured in a real time electrical readout. GMR biosensor detected as low as 1.5 × 102 TCID50/mL virus and the signal intensity increased with increasing concentration of virus up to 1.0 × 105 TCID50/mL. This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 103 to 105 TCID50/mL.
Highlights
Influenza viruses belong to the family Orthomyxoviridae, which are enveloped single strand negative sense RNA viruses with segmented RNA genome
To validate the Influenza A viruses (IAVs) specific antibodies to use in giant magnetoresistance (GMR) biosensor assay, we performed antigen capture enzyme linked immunosorbent assay (ELISA) using human or swine isolates of IAV
A sensitive GMR biosensor was developed for the detection of influenza virus
Summary
Influenza viruses belong to the family Orthomyxoviridae, which are enveloped single strand negative sense RNA viruses with segmented RNA genome. Based on their matrix (M) and nucleoprotein (NP), influenza viruses are classified into type A, B, or C. IAV is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV) and birds (avian influenza virus or AIV). Sensitive, and reliable method for detection of IAV in the environment, tissues and body fluids is important for controlling the infection and reducing the impact of possible influenza pandemic by early detection and rapid intervention. Detection of viral antigens and serological test for antibody detection are either poor in specificity
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.