Abstract
Seed purity identification is an indispensable process before seeds are used in production and is the key to ensuring seed quality. Developing an accurate, effective, inexpensive and convenient method for Wax gourd seeds purity identification can provide technical support for seed quality. The co-dominant primers of XD-18 and XD-99 were chosen to test the seed purity of ‘Jinyuan 2’, ‘Jinyuan 3’ and ‘Lvxianzi 2’ 3 Wax Gourd hybrids in this research. The F 2 generations of Wax Gourd hybrids was used to analyze whether the 2 SSR markers were in accordance with Mendelian separation law. If it is in accordance with Mendelian separation, then 2 pairs of primers were used to carry out SSR molecular marker purity identification of hybrid seeds of Wax gourd, and a comparison experiment was performed between the SSR molecular marker purity identification and the field planting purity identification. The results showed that the 2 SSR markers were in accordance with the Mendelian separation law of 1: 2: 1 in the F 2 generation population and SSR molecular marker purity identification of Wax gourd hybrid seeds was highly consistent with the field planting purity identification. Therefore, this study provides a more accurate technical system for the purity identification of Wax gourd seeds and other crop hybrid seeds.
Highlights
The wax gourd (Benincasa hispida Cogn.) is an annual climbing plant of Cucurbitaceae
The results showed that the 2 SSR markers were in accordance with the Mendelian separation law of 1: 2: 1 in the F2 generation population and SSR molecular marker purity identification of Wax gourd hybrid seeds was highly consistent with the field planting purity identification
The results showed that the SSR primers XD-18 and XD-19 showed co-dominant characteristics, and the F1 generation showed complementary bands of parents, which could effectively distinguish the hybrids from their parents, indicating that the two SSR markers could be used to identify the hybrid seed purity of wax gourd
Summary
The wax gourd (Benincasa hispida Cogn.) is an annual climbing plant of Cucurbitaceae. SSR (Simple sequence repeat) molecular markers have the advantages of co-dominance, good repetitiveness, high polymorphism, and are not affected by conditions such as external environment, tissue and development It has been repeatedly used in rice (Moorthy et al, 2011), broccoli (Yu et al, 2013), cauliflower (Zhao et al, 2012), melon (Ai et al, 2005; http://en.cnki.com.cn/Article_en/CJFDTotal-FZZW2006S2005.htm), watermelon (Li et al, 2015, Chinese Agricultural Science Bulletin, 31(33): 177-185; Lu et al, 2018), maize (Li et al, 2018), cotton (Selvakumar et al, 2010; Fu et al, 2017), sunflower (Pallavi et al, 2011) and other crops hybrid seed purity identification. It was compared with grow out test (GOT) to provide a new and accurate technical system for the identification of hybrid seed purity
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