Abstract
The aim of this study is to clarify the effects of crude garlic feeding (Allium sativum) on somatic and germ cells on adult rat. Twenty four adult male rats were divided into four groups: group 1 (untreated) and groups 2, 3 and 4 were fed for 30 days with 5 g, 10 g and 30 g/100 g of standard diet, respectively. Through TUNEL, immunostaining and Western blotting approaches, we showed that crude garlic feeding induced apoptosis in testicular germ cells (spermatocytes and spermatids) from rats fed with 10 g and 30 g of A. sativum. This cell death process was characterized by increased levels of active caspase-3 but not caspase-6. Expression of the caspase inhibitors c-IAP1 and c-IAP2 was increased at all doses of garlic, while expression of apoptosis inducing factor mitochondrionassociated (AIFM1) was unchanged. Moreover, expression of the IAP inhibitor Smac/DIABLO was increased from groups 3 and 4. In summary, we showed that feeding with crude garlic, for one month at high doses; induce apoptosis in testicular cells specially spermatocytes and spermatids but not somatic cells.
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