Geographical Traceability of &amp;lt;i&amp;gt;Clinacanthus nutans&amp;lt;/i&amp;gt; with Near-Infrared Pectroscopy and Chemometrics

Fengyan Yu,Furong Huang,Guiliang Tan,Han Song,Yi Qi,Jinfang Ma,Maoxun Yang

doi:10.4236/ajac.2022.132006

Abstract

In this study, a seed origin discrimination model for Clinacanthus nutans was developed. First, 81 C. nutans samples from three seed origin locations were collected, and their Near-Infrared (NIR) spectra were obtained. Next, Principal Component Analysis (PCA) was performed on the NIR spectra of the 81 C. nutans samples. Then, MSC (multiplicative scatter correction), SNV (standard normal variate), first derivative, and second derivative pre-treatments of the C. nutans spectra were performed and combined with the Support Vector Machine (SVM) algorithm for modelling and analysis. Among these methods, first-order derivative pre-treatment achieved the best SVM model effectiveness, with a training set accuracy of 93.44% (57/61) and a test set accuracy of 85.00% (17/20). In order to further improve the discrimination accuracy of the model, three optimization algorithms Grid Search (GS), Genetic Algorithm (GA), and Particle Swarm Optimization (PSO) were employed to identify the best c and g parameters for the SVM model. The results demonstrated that the PSO optimization algorithm yielded the best parameters of c = 0.8343, g = 57.8741, with corresponding model training set the accuracy of 96.36% (60/61) and test set the accuracy of 95.00% (20/21). Therefore, developing a seed origin classification model for C. nutans based on NIR spectroscopy combined with chemometrics is feasible and has the advantages of being simple, rapid, and green.

Highlights

Clinacanthus nutans (C. nutans), known as the alligator flower, or the Sabah snake grass, is a plant belonging to the genus Clinacanthus in the family of Acanthaceae
The results demonstrated that the Particle Swarm Optimization (PSO) optimization algorithm yielded the best parameters of c = 0.8343, g = 57.8741, with corresponding model training set the accuracy of 96.36% (60/61) and test set the accuracy of 95.00% (20/21)
NIR spectroscopy data were collected from 81 C. nutans samples from three geographic locations: Malaysia, Hainan (China), and Guangxi (China)

Summary

Introduction

Clinacanthus nutans (C. nutans), known as the alligator flower, or the Sabah snake grass, is a plant belonging to the genus Clinacanthus in the family of Acanthaceae. A flurry of research carried out about the chemical composition of C. nutans, confirmed that they are rich sources of flavonoids, phenolics, steroids, triterpenoids, cerebrosides, glycoglycerolipids, glycerides, and sulfur-containing glycosides, which make them a useful folk medicine and interesting healthy food [4] [5] [6] [7]. Various compositional and health studies concluded that C. nutans herbal tea has considerable potential as the potential natural antioxidant source. Accurate determination of the origin of C. nutans seeds is scientifically important and has application in relevant medicines, health food materials, as well as establishing product quality standards [8]

Methods

Results

Conclusion