Genotyping of rs228666 SNP of the human gene ACE2 in mild, moderate and severe COVID-19 patients.

Abstract

The ACE2-converting enzyme has been identified as the specific receptor for coronavirus, but the effect of the ACE2 gene polymorphism is still unknown, so the expression of human ACE2 independently may affect the susceptibility to infection with COVID-19. Coronavirus is an infectious and pandemic disease caused by it. To evaluate the association between the ACE2 gene polymorphism and the severity of COVID-19 infection, we used PCR polymerase reaction and polymorphism methods to examine 80 patients classified into severe infection based on symptoms and 80 control factors. Results demonstrated statistical significance in this high-risk group and the function of rs228666 SNP in the risk of infection with the independent ACE2 gene rs228666. Individuals who have the variation A allele may be more vulnerable to infection than those who carry G in a condition with the GA genotype when compared to other genotypes GG and GA. In contrast, the AA genotype was not significant. Coronavirus susceptibility and disease severity were related to inflammation and genetic polymorphism, while there was no clear evidence linking this rs228666 SNP to the severity of COVID19 infection. At the 0.01 % probability level, the prediction of the GG genotype for control was not significant. In the case of mild infections, the ACE2 rs228666 SNP genotype was found to be a protective factor for infection with COVID-19 illness in Iraqi patients. Furthermore, patients with this GA genotype are more likely to develop COVID-19 illness than the other genotypes, although the AA genotype had no significant effect on this disease. Individuals with the A allele are also more likely to be infected with COVID-19 than those with the G allele, according to the findings. The GG genotype of the ACE2 rs228666 SNP was substantially more significant at the 0.01 % probability level in the medium cases compared to the control group, showing that the GG genotype of the ACE2 rs228666 SNP is a protective factor against the incidence of infection. With COVID-19 in Iraqi patients, however, the values of the AA genotype are not significant, implying that an individual who carries the A allele is more likely to get COVID-19 disease than those who possess the GG allele. Keywords: Coronavirus, SNP, ACE2.