Abstract
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have been reported among C. gigas in Europe in relation to the detection of a variant of OsHV-1, called μVar. Since 2009, this variant has been mainly detected in France. These results raise questions about the emergence and the virulence of this variant. The search for association between specific virus genetic markers and clinical symptoms is of great interest and the characterization of the genetic variability of OsHV-1 specimens is an area of growing interest. Determination of nucleotide sequences of PCR-amplified virus DNA fragments has already been used to characterize OsHV-1 specimens and virus variants have thus been described. However, the virus DNA sequencing approach is time-consuming in the high-scale format. Identification and genotyping of highly polymorphic microsatellite loci appear as a suitable approach. The main objective of the present study was the development of a genotyping method in order to characterise clinical OsHV-1 specimens by targeting a particular microsatellite locus located in the ORF4 area. Genotyping results were compared to sequences already available. An excellent correlation was found between the detected genotypes and the corresponding sequences showing that the genotyping approach allowed an accuraté discrimination between virus specimens.
Highlights
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order [1]
Since 2008, massive mortality outbreaks have been reported among C. gigas spat in several farming areas in Europe [6,7,8,9,10,11] in relation to the detection of a newly described OsHV-1 variant called μVar [7]
The reference type and the variant μVar were detected in association with mortality outbreaks in 2008 in France, virus detection since 2009 has mainly concerned the μVar variant [7,10,12]
Summary
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order [1]. The reference type (a viral specimen collected in France in 1995 during a mortality event affecting C. gigas larvae, GenBank accession number AY509253) and the variant μVar were detected in association with mortality outbreaks in 2008 in France, virus detection since 2009 has mainly concerned the μVar variant [7,10,12]. These results raise questions about the emergence and the virulence of the μVar variant. In light of the genetic diversity of OsHV-1, the search for associations between specific virus genetic markers and clinical symptoms is of great interest
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