Genotyping and virulence genes of Helicobacter pylori isolated from Patients with Gastroenteritis in Al-Diwaniyah Province/Iraq

Abstract

The frequency distribution of Helicobacter pylori approximately 20 and 40% in developed nations and about 90%in developing countries. Diseases related to Helicobacter pylori is detected by a number virulence factors. So, the aim of this work is to detect virulence gene; VACAm1 (Vaculating Cytotoxin Type Am1), VACAm2 (Vacuolating Cytotoxin Type Am2), and IceA2 (Induced by Contact with Epithelium Gene A2) in Helicobacter pylori isolates from patients with gastroenteritis symptoms using the molecular method (PCR). This study was conducted in Al-Diwaniyah Governorate / Iraq, and it included 40 patients, their ages ranged between 11 and 56 years, of both sexes. The samples were collected from October 2021 to February 2022. It depended on the diagnosis of the specialist doctor in diagnosing patients with gastroenteritis. Samples were collected from the Gastrointestinal Center at Al-Diwaniyah Teaching Hospital and the Internal Medicine Consultation, 20 stool samples were collected and placed in a sterile container and preserved with a phosphate buffer saline solution and 20 Tissue biopsy samples were taken from the patients’ stomachs where biopsies excised from the gastric antrum using endoscopic forceps and preserved with normal saline solution.