Abstract
Aim : This study was carried out to determine the presence of TEM and SHV genes in extended-spectrum ?-lactamase (ESBL) producing Klebsiella pneumoniae . The study was also aimed to compare results of PCDDT and genotypic methods. Information on molecular types of ESBL positive Klebsiella species is less from India; lesser still from this area of Marathwada, a part of Maharashtra state. Materials and Methods: A total of 340 strains of Klebsiella pneumoniae were selected for the study from June 2013-December 2013. Kirby Bauer disk diffusion method was performed to determine the antibiotic resistance pattern. Screened for ESBL and confirmed by phenotypic confirmatory disc diffusion test (PCDDT). 100 randomly selected isolates were investigated for the presence of TEM and SHV genes via Polymerase chain reaction (PCR) using two different sets of primers. Multiplex PCR was also performed for the same. Results: Phenotypic confirmatory test was able to detect ESBL production in 90% of Klebsiella pneumoniae isolates. Among the two ESBL genotypes, the most prevalent genotype was found to be TEM. Majority of ESBL producing isolates possess both ESBL genes. Conclusion: Multiplex PCR can be used as a rapid method to identify common genes (TEM and SHV) responsible for extended spectrum beta lactamase production in Klebsiella pneumoniae. It will prove valuable for surveillance and for determining the line of treatment against drug resistant organisms, thus saving precious time and resources. PCDDT results correlated with genotypic method in all the tested strains.
Highlights
Extended-spectrum β-lactamases (ESBLs) continue to be a major problem in clinical setups the world over, conferring resistance to the expandedspectrum cephalosporins.[1]
All the samples were processed by standard methods.[4][5][6] Samples obtained were screened for resistance to 3rd generation Cephalospoirins (3GC).The ESBL status was determined by phenotypic confirmatory disc diffusion test (PCDDT)
It was observed that many (≥80%) of our isolates of Klebsiella pneumoniae were resistant to third generation Cephalosporin’s and other antibiotics
Summary
Extended-spectrum β-lactamases (ESBLs) continue to be a major problem in clinical setups the world over, conferring resistance to the expandedspectrum cephalosporins.[1] The vast majority of Klebsiellae infections are associated with hospitalization. Klebsiella species primarily attack immunocompromised individuals who are hospitalized and suffer from severe underlying diseases. Nosocomial Klebsiella infections are caused mainly by Klebsiella pneumoniae, the medically most important species of the genus.[2]. Extended spectrum β-lactamases (ESBL’s) are rapidly evolving group of β-lactamase enzymes produced by Gram negative bacteria. These enzymes have been derived from TEM and SHV genes by mutations and have been well described in Enterobacteriaceae especially
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Biomedical and Advance Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
