Genotype of Cryptosporidiumspp. isolated from human of Al-Qadisiyah province Iraq.

Abstract

Abstract:
 The current study included examination of 100 stool sample from human was collected from AL-Qadisiyah province, from September 2018 until February 2019.the Microscopic examination result showed that oval or spherical shaped with dark pink color or red oocyst on blue ground and 22(22%) positive sample out 100 case . It was recorded that the highest rate 30% (3/10) was seen in September , but the lowest infection rate 13.3% (2/15) was seen in the month of December with no significant differences at level (p<0.05.) regarding to age the high rate of infection 26.31%(10/38)was found in the group less than a year , but the lowest infection rate was observed in the group( >12 years). There is no significant differences at p<0.05.between man and women . In this study the N- PCR in molecular examination were used ,the positive sample was13 (59.09%) out of 22 stool sample. Sequencing of a fragment of the (18s rRNA) gene (834 bp) that separated from many different area in AL-Qadisiyah government recorded (75%)9/12 samplerelated to NCBI – Blast Cryptosporidium hominis isolates ,( 25%) 3/12 sampledisplay closed related to NCBI –Blast Cryptosporidium parvum .In Conclusion The current study concluded that phylogenetic tree and the homology sequences identity gives a clear differentiation of the types of Cryptosporidium parasites which can be isolated at high rates of human in AL- Diwaniyah province, which is likely to lead to an outbreak of Cryptosporidiosis in this province.