Genotoxicity Assessment of an Organophosphate Insecticide, Ethion Employing Mosquito Genome (Diptera:<i>Culicidae</i>)

Abstract

The preferential motives of the present exploration are attributed to the genotoxicity evaluation of an organophosphate pesticide, ethion on the heredity material of Culex quinquefasciatus , exposed to sublethal aliquot, LC 20 , for the continuation of 24 h, implementing two in vivo approaches viz., the testicular chromosomal aberrations analysis and dominant lethal test (DLT). Mutagenic, as well as clastogenic consequences of ethion on germinal chromosomes were observed, specifically through relatively elevated incidences of nondisjunctions during pole-wise movement of chromosomes, excessively condensed heterochromatinizations regions, chromosomal fusions due to stickiness, aneuploidy eventually by expressed by hypoploidy, lags of whole or chromosomal fragments and furthermore, pole to pole extended chromosomal bridges, in comparison to control population, with statistical corresponding values 26.03 ± 15.5 in treated stock as against 12.67 ± 3.8 in control groups. For DLT, treated male imagoes were allowed to crossmate with normal females to procure egg rafts and enhanced percentage frequency of unhatched eggs corresponds to value 12.39 ± 3.00 in treated stocks as correlated to 4.23 ± 1.12 in control stocks. Subsequently, statical examination of procured data, from both executed parameters, demonstrated significant outcomes, when scrutinized through Student's t-test (P < 0.05). The overall conclusion indicated that the induced genotoxicity of ethion in germinal chromosomes could be the probable reason for dominant lethality observed during DLT.