Abstract
Objective: Sequencing of mouse Tbx2 gene and observing the expression of Tbx2 gene in normal and malignant melanophore. Methods: The PCR products of Tbx2 cDNA were cloned into pUC18 vector and sequenced. The normal and malignant melanocytes were used to extract total RNA. The expression of Tbx2 gene was detected by RT-PCR. Results: The Tbx2 genome is composed of seven exons and six introns. No expression of Tbx2 gene in the normal melanocytes was noted, but all malignant melanocytes showed expression of Tbx2 gene. Conclusion: The observation showed the analysis of the genomic structure of mouse Tbx2. Tbx2 plays a critical role during the development of the malignant melanophore.
Paper version not known (
Free)
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call