Genomic polymorphism of human papillomavirus type 52 in women from Northeast China.

Zhengrong Sun,Qiang Ruan,Chao Liu,Weiqiang Zhou,Jianhua Liu,Lianxia Yang,Guili Wang,Zhitao Lu

doi:10.3390/ijms131114962

Zhengrong Sun, Qiang Ruan + Show 6 more

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https://doi.org/10.3390/ijms131114962

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Abstract

Human papillomavirus (HPV) 52 is an oncogenic HPV type prevalent in Asia. The aim of the study was to analyze HPV 52 genetic variations in women from Northeast China. To explore the intratypic variants of HPV 52, the genomic regions of L1, E6, E7 and long control region (LCR) of HPV 52, which have been identified in women from Northeast China by HPV GenoArray test, were analyzed. Twenty-five mutations were identified in the regions examined. Of the mutations found in the L1 gene, three novel nonsynonymous mutations of C5640T, A5641T and G5642A were located within the region that encodes the binding domain of neutralizing antibodies against HPV 52. Although four variations were identified in HPV 52 E6 and E7 genes, no significant association was found between the mutations and the cytological lesion of the patients. Eight mutations, including a novel CTT7681–7683 deletion, found in the LCR of HPV 52 encompassed the known transcription binding sites, which may possibly affect the transcription of the oncogenic genes of E6 and E7. The most prevalent HPV 52 variant in women from northeastern China belongs to clade L1-LN-A. The genetic variations of HPV 52, including three novel nonsynonymous mutations of C5640T, A5641T and G5642A in the L1 gene and a novel CTT7681–7683 deletion in the LCR, were first documented in strains from women in Northeast China. The statistical result showed no associations between the variants and the severities of the infected women. These findings provide new data regarding gene variations of HPV 52.

Highlights

Oncogenic human papillomavirus (HPV) is a major cause of cervical cancer
For the HPV types found from the study population, 8.8% (72/815) were solely infected by HPV 52
L1, E6, E7 and long control region (LCR) genes were performed on all the single HPV 52 positive samples where sufficient materials were available

Summary

Introduction

Oncogenic human papillomavirus (HPV) is a major cause of cervical cancer. HPV 16 and HPV 18 are highly prevalent in all regions of the world [1,2], followed by HPV 31, 33. Accumulated studies have shown that HPV 52 and 58 are relatively more prevalent among. HPV-positive women from Asia than in other places with only 11.5%–28% of prevalence across the full spectrum of cervical neoplasia [3,4,5]. It has been estimated that worldwide HPV prevalence is 10.4% in women with normal cervical cytology [6], but less than 1% of the infected women develop malignant lesions [7]. It is clear that persistent infection with specific high-risk HPV types is a strong marker for progressive CIN disease [8]. Recent studies have revealed that persistence of high-risk HPV infection might be associated with virus intratype variants. Concerning HPV intratype variants, the most extensive studies have been conducted on HPV16 and HPV18 [10,11]. Few data about HPV intratype variants has been achieved so far

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