Abstract
Aeromonas salmonicida subsp. salmonicida is a Gram-negative bacterium that causes furunculosis, a fish disease claiming substantial economic losses in the aquaculture industry. Major challenges exist in monitoring and controlling fish infections in aquaculture farms. Development of management practices to improve the sustainability of fish farming with disease prevention necessitates studies using well-defined systems and well-characterized bacterial isolates. Even though several A. salmonicida subsp. salmonicida genomes have been completely assembled and thoroughly annotated, in vivo pathogenicity data are lacking. Here we present A. salmonicida subsp. salmonicida 890054 as a prototype strain for standardized furunculosis challenges with survival data. Computational analysis of sequencing results provided a complete circular genome with annotations of plasmids carrying virulence factors, antimicrobial resistance, and secondary metabolite coding genes. The analysis also revealed the presence of an IncU plasmid distinct from other IncU plasmids previously associated with Aeromonas.
Highlights
The Gram-negative bacterium Aeromonas salmonicida subsp. salmonicida is the etiologic agent of furunculosis, a disease affecting salmonids and being a substantial burden in the aquaculture industry (Bartkova et al, 2017)
The hybrid infection protocol using A. salmonicida subsp. salmonicida strain 890054 led to daily mortalities without depleting fish stocks; the mortality rate was roughly 50% 37 dpi (Figure 1B)
A single death event in the Baseline control group could not be linked to a positive A. salmonicida subsp. salmonicida culture from the kidney stab and was censored prior to plotting survival curves
Summary
The Gram-negative bacterium Aeromonas salmonicida subsp. salmonicida is the etiologic agent of furunculosis, a disease affecting salmonids and being a substantial burden in the aquaculture industry (Bartkova et al, 2017). Well-defined systems are required for the Genomic Perspectives on Aeromonas salmonicida 890054 study of freshwater fish culture and disease prevention. Salmonicida isolates have been used in various in vivo pathogenicity studies especially in vaccine development (Marquis and Lallier, 1989; Garduño et al, 1993; Marsden et al, 1996; Marana et al, 2017). Most of those studies used different strains of A. salmonicida subsp. This may lead to difficulties in repeating and generalizing results, in part due to the tremendous genomic diversity within the accessory genome of this subspecies (Attéré et al, 2017; Massicotte et al, 2019; Vincent et al, 2020)
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