Abstract
An analysis of Drosophila Su(Hw) binding allowed the identification of new, isolated, binding sites, and the construction of a new binding site consensus. Together with gene expression data, this supports a role for Su(Hw) in maintaining a constant genomic architecture.
Highlights
Insulator elements are proposed to play a key role in the organization of the regulatory architecture of the genome
The full array design for the Adh region is described in the report by Birch-Machin and coworkers [26] and the array has been supplemented with other selected Drosophila genomic sequences; of particular http://genomebiology.com/2007/8/8/R167
We assessed the immunopurifications by standard polymerase chain reaction (PCR) assays using specific primer pairs and could demonstrate clear enrichment for known Su(Hw) targets, the gypsy insulator, and the 1A-2 site in the achaetescute region [20,21], but no enrichment for a Gpdh control fragment
Summary
Insulator elements are proposed to play a key role in the organization of the regulatory architecture of the genome. In Drosophila, one of the best studied is the gypsy retrotransposon insulator, which is bound by the Suppressor of Hairy-wing (Su [Hw]) transcriptional regulator. Insulator elements are proposed to play a key role in the organization of transcriptional regulation within the eukaryotic genome [1,2]. They were first identified as DNA sequences that regulate interactions between promoter and enhancer elements, and are operationally defined as sites that, when positioned between an enhancer and a promoter, block this enhancer/promoter interaction while still allowing. Http://genomebiology.com/2007/8/8/R167 the enhancer to operate on other promoters This function suggests that insulators act to organize independent gene regulatory domains in the genome by preventing inappropriate enhancer/promoter interactions. In addition to Su(Hw), the gypsy insulator complex includes the BTB/ POZ domain proteins Mod(mdg4) 2.2 [7,8] and Centrosomal Protein 190 [9], together with dTopors (a ubiquitin ligase) [10]
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