Abstract
Artemisia annua is an important medicinal plant, used for curing various diseases especially malaria. It secretes verities of secondary metabolites which hinders in the DNA extraction. This investigation describes an efficient DNA extraction protocol for A. annua based upon the Cetyl Trimethyl Ammonium Bromide (CTAB) extraction method without using hazardous chemicals i.e. liquid nitrogen and phenol. The developed protocol is simple, reliable and operative in normal laboratory condition yielding intact DNA having good quantity (502.7 to 1288.5 ng/µl) and quality (A260/280 ratio - 1.82 to 1.85) in two working days. Proper amplification of extracted DNA indicates its suitability for the various molecular biological applications. Int J Appl Sci Biotechnol, Vol 4(4): 448-451
Highlights
Artemisia anuua L. is a traditional Chinese medicinal plant well known for its bio-active compound artemisinin–an antimalarial drug
Genomic DNA of A. annua was isolated without using liquid nitrogen and phenol in two subsequent days
The first day extracted DNA was brown in colour having DNA concentration from 742.6 to 1556.9 ng/μl with A260/A280 ratio of 0.83 to 0.95, while the DNA concentration of second day extracted DNA was varied from 502.7 ng/μl to 1288.5 ng/μl with A260/A280 ratio of 1.82 to 1.85 (Table 1)
Summary
Artemisia anuua L. is a traditional Chinese medicinal plant well known for its bio-active compound artemisinin–an antimalarial drug. There are many DNA extraction protocol for A. annua has been developed by using liquid nitrogen and/or phenol (Kumar et al, 2011; Lakshmi et al, 2012). There is a need for development of DNA extraction method for normal laboratory conditions, which avoid the use of liquid nitrogen and phenols.
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