Genomic DNA Isolation from High Polyphenolic Content Grewia asiatica L. Leaf Without Using Liquid Nitrogen

Abstract

Grewia asiatica (Grewoideae family) is also known as Phalsa, used for the medicinal purpose has a high content of polysaccharides and polyphenols together with flavonoids and other secondary metabolites which obstructs their pure genomic DNA extraction. Although, there are various good quality DNA extraction protocols, extracting DNA from a high phenolic content plant is a challenging task. The present study describes an efficient and rapid DNA isolation protocol by optimizing cetyl trimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP) and sodium dodecylsulphate (SDS) cocktail specifically for the extraction of DNA from polyphenolic leaf of G. asiatica without using liquid nitrogen. Extracted genomic DNA has high purity index (1.95) examined by A260/A280 ratio. The presence of good quality DNA was also evident by gel electrophoresis. The optimized DNA extraction method is efficient for both fresh and old tough leaves with high polyphenolic contents. The achievement of this method in extracting high quality of genomic DNA demonstrated the broad applicability of this method.