Genomic composition of microbiol gingival sulcus and periodontal pockets in young persons

Abstract

Relevance. Risk factors of local importance plays a crucial role in the development of inflammatory periodontal diseases, but the profile of representation and the role of origin microbial markers continues to be refined, what explains the increasing interest by the metagenomic studies. Purpose. To compare the genomic composition of the microbiota of the periodontal sulcus and periodontal pocket by healthy patients with inflammatory periodontal diseases living on the territory of Kazan, the Republic of Tatarstan. Materials and methods. The study included 25 young people (11 boys, 14 girls) aged 18-19 years, with inflammatory periodontal diseases (chronic generalized catarrhal gingivitis (12 people), chronic generalized periodontitis of mild severity (13 people)). The control group consisted of 11 donors without inflammatory periodontal disease.Results. In the present study structures of microbial communities of periodontal spaces has been analyzed with using the sequencing of fragments of bacterial 16s rRNA genes (regions V3 and V4). Results of the analysis allowed to get a real idea of its composition and to determine both known and previously undefined uncultivated phylotypes. Conclusions. It was shown that in the group of the patients with inflammatory periodontal diseases there were identified 183 phylotypes at the level of genus (Mogibacteriacea, TM7 3, Rs–045, Dethiosulfovibrionaceae) relating to 17 phyls (phylum), that is a synonym of type in taxonomy (taxon between Kingdom and class). By the patients with chronic generalized periodontitis of mild severity, it was not possible to reliably isolate the phylotypes present in increased amounts in relation to chronic catarrhal gingivitis; in relation to control – there was a statistically significant increase in the proportion of families Porphyromonadaceae, Peptostreptococcaceae and the proportion of genera Dialister, Filifactor, Parvimonas, Tannerella, Treponema.