Abstract
Plant P-type H+-ATPase (P-ATPase) is a membrane protein existing in the plasma membrane that plays an important role in the transmembrane transport of plant cells. To understand the variety and quantity of P-ATPase proteins in different cotton species, we combined four databases from two diploid cotton species (Gossypium raimondii and G. arboreum) and two tetraploid cotton species (G. hirsutum and G. barbadense) to screen the P-ATPase gene family and resolved the evolutionary relationships between the former cotton species. We identified 53, 51, 99 and 98 P-ATPase genes from G. arboretum, G. raimondii, G. barbadense and G. hirsutum, respectively. The structural and phylogenetic analyses revealed that the gene structure was consistent between P-ATPase genes, with a close evolutionary relationship. The expression analysis of P-ATPase genes showed that many P-ATPase genes were highly expressed in various tissues and at different fiber developmental stages in G. hirsutum, suggesting that they have potential functions during growth and fiber development in cotton.
Highlights
H+ -ATPase is a membrane protein that is widely present in the plant plasma membrane and various endomembranes, and plays an important role in cell metabolism
We first queried the specific domains of Arabidopsis AHA10 (PF00122, PF00702, and PF00690) on the Pfam website and used the amino acid sequences of these domains to search for candidate genes in the local databases of G. arboretum, G. raimondii, G. barbadense and G. hirsutum, which were previously established
P-ATPase genes were found in G. raimondii and 53 P-ATPase genes were found in G. arboretum, similar to those in Arabidopsis and rice, containing 46 and 43 P-ATPase genes, respectively
Summary
H+ -ATPase is a membrane protein that is widely present in the plant plasma membrane and various endomembranes, and plays an important role in cell metabolism. H+ -ATPases are currently divided into three types according to structure, namely the vacuolar-type. H+ -ATPases (V-ATPases), H+ -pyrophosphatases (H+ -PPases) and P type H+ -ATPases (P-ATPase). The. H+ -PPases is located in vacuolar or endomembranes; the vacuole H+ -ATPase is a V-type ATPase; and the plasma membrane H+ -ATPase belongs to the P-type ATPase. As a P-type pump, P-ATPase can generate and maintain the electrochemical gradient of H+ on both sides of the cell membrane by decomposing intracellular ATP and providing energy for the transmembrane transport of various nutrients and ions [1,2]. 10 subfamilies of P-ATPase have been identified according to their transport of various ions, heavy metals and possibly lipids: P1B (heavy metal ion ATPases [HMA]), P2A (endoplasmic reticulum Ca2+ ATPase [ECA]), P2B (autoinhibited Ca2+ ATPase [ACA]), P3A (autoinhibited H+ ATPase (AHA)), P4 (putative aminophospholipid ATPase [ALA]), P5 (unknown ATPase), P1A (K+ ATPase), P2C
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