Abstract
This study aimed to genetically characterize two fully-sequenced novel IncFII-type multidrug resistant (MDR) plasmids, p0716-KPC and p12181-KPC, recovered from two different clinical Klebsiella pneumoniae isolates. p0716-KPC and p12181-KPC had a very similar genomic content. The backbones of p0716-KPC/p12181-KPC contained two different replicons (belonging to a novel IncFII subtype and the Rep_3 family), the IncFIIK and IncFIIY maintenance regions, and conjugal transfer gene sets from IncFIIK-type plasmids and unknown origins. p0716-KPC and p12181-KPC carried similar three accessory resistance regions, namely ΔTn6209, a MDR region, and the blaKPC-2 region. Resistance genes blaKPC-2, mph(A), strAB, aacC2, qacEΔ1, sul1, sul2, and dfrA25, which are associated with transposons, integrons, and insertion sequence-based mobile units, were located in these accessory regions. p0716-KPC carried two additional resistance genes: aphA1a and blaTEM-1. Together, our analyses showed that p0716-KPC and p12181-KPC belong to a novel IncFII subtype and display a complex chimeric nature, and that the carbapenem resistance gene blaKPC-2 coexists with a lot of additional resistance genes on these two plasmids.
Highlights
Enterobacteriaceae, Pseudomonas, and Acinetobacter species, with K. pneumoniae being the most common species harboring blaKPC genes[1, 2]
IncFIIY-type plasmid from Klebsiella oxytoca isolated in China[6]
This work presents the complete sequences of two novel multidrug resistant (MDR) plasmids, p0716-KPC and p12181-KPC, from K. pneumoniae strains isolated from China (Table 1)
Summary
Enterobacteriaceae, Pseudomonas, and Acinetobacter species, with K. pneumoniae being the most common species harboring blaKPC genes[1, 2]. KPC confers resistance or decreased susceptibility to almost all β-lactams, and KPC-producing isolates are often resistant to many other non-β-lactam drugs because of the co-occurrence of blaKPC with other classes of resistance gene. This multidrug resistance (MDR) leaves few available options for antimicrobial treatment, and thereby results in high mortality rates[3]. IncFIIY-type plasmid from Klebsiella oxytoca isolated in China[6]. Highly unusual, these backbone components can function together to promote the replication and stability of pKPC-LK30 in K. pneumoniae. Co-occurrence of blaKPC-2 (carbapenem resistance) with mph(A) (macrolide resistance), strAB and aacC2 (aminoglycoside resistance), qacEΔ1 (quaternary ammonium compound resistance), sul[1] and sul[2] (sulphonamide resistance), and dfrA25 (trimethoprim resistance) was observed in both plasmids
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