Abstract
Nattokinase is a serine protease which is produced by Bacillus subtilis and exhibits vigorous fibrinolytic activity. Nattokinase is useful for preventing and treating thromboembolic diseases. In this study, the effect of ultraviolet combined 60Co-γ ray on B. subtilis were investigated, and the mechanism of NK production enhancement was elucidated at molecular level. There was obvious differences of NK production and growth characteristic between mutant (JNFE1126) and wild type (JNFE0126) B. subtilis strains, the NK production of B. subtilis JNFE1126 increased by 2 times compared to the wild strain. By comparative genomics analysis, 28 single nucleotide polymorphisms (SNPs), and 15 insertion deletions (Indel) were found. In terms of the transcriptional data, the results revealed there were 1425 genes with differential expression of fold change>=2. Functional categorization and analysis of modulated genes of interlinked pathways pointed out plausible nattokinase production in JNFE1126. Such as a dependency on amino acid metabolism for generating energy, and promoted transcription and extracellular transport of nattokinase. The upregulation expression of NadB, the positive regulatory genes (ComA and ComP), SecY and YidC in JNFE1126. Overall, the study provided an understanding of the molecular basis linked to high nattokinase production in JNFE1126.
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