Genomic analysis of intestinal flora and liver genes in mice with circadian rhythm disorders fed with flavonoids from Sedum aizoon L.

Abstract

A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L. has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are often accompanied by changes in intestinal flora, and flavonoids that can regulate intestinal flora may be an effective way to prevent and regulate circadian rhythm disorders. In this study, we established a humanized circadian rhythm mouse model to explore the regulatory mechanism of flavonoids from Sedum aizoon L.(FSAL) on circadian rhythm disorders in mice. The results after feeding FSAL for 4 weeks showed that FSAL improved the imbalance of intestinal microbial structure caused by circadian rhythm disorders, thus regulating alanine, aspartate and glutamate metabolism, histidine metabolism and other metabolites and substances related to metabolism. The analysis of liver transcriptome showed that FSAL significantly regulated retinol metabolism, peroxisome proliferator-activated receptors(PPARs) pathway, and hepatitis C pathway in mouse liver. Therefore, this study obtained the conclusion that FSAL can regulate intestinal flora, metabolites and liver genes to improve circadian rhythm disorders and maintain mouse health.