Genome-wide identification and expression profile analysis of the NAC transcription factor family during abiotic and biotic stress in woodland strawberry.

He Zhang,Hao Kang,Jinji Pu,Yanxiang Qi,Chulian Su,Xiaomei Liu

doi:10.1371/journal.pone.0197892

He Zhang, Hao Kang + Show 4 more

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https://doi.org/10.1371/journal.pone.0197892

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Abstract

The NAC transcription factors involved plant development and response to various stress stimuli. However, little information is available concerning the NAC family in the woodland strawberry. Herein, 37 NAC genes were identified from the woodland strawberry genome and were classified into 13 groups based on phylogenetic analysis. And further analyses of gene structure and conserved motifs showed closer relationship of them in every subgroup. Quantitative real-time PCR evaluation different tissues revealed distinct spatial expression profiles of the FvNAC genes. The comprehensive expression of FvNAC genes revealed under abiotic stress (cold, heat, drought, salt), signal molecule treatments (H2O2, ABA, melatonin, rapamycin), biotic stress (Colletotrichum gloeosporioides and Ralstonia solanacearum). Expression profiles derived from quantitative real-time PCR suggested that 5 FvNAC genes responded dramatically to the various abiotic and biotic stresses, indicating their contribution to abiotic and biotic stresses resistance in woodland strawberry. Interestingly, FvNAC genes showed greater extent responded to the cold treatment than other abiotic stress, and H2O2 exhibited a greater response than ABA, melatonin, and rapamycin. For biotic stresses, 3 FvNAC genes were up-regulated during infection with C. gloeosporioides, while 6 FvNAC genes were down-regulated during infection with R. solanacearum. In conclusion, this study identified candidate FvNAC genes to be used for the genetic improvement of abiotic and biotic stress tolerance in woodland strawberry.

Highlights

Transcription factors containing the highly conserved NAC domain (NAM-ATAF1/2-CUC) in the N-terminal region compromise is one of the largest transcription factors families in plants [1,2,3]
Genome-wide analyses have identified a large number of NAC family members in several species, including 117 NAC genes in the model plant Arabidopsis thaliana [7], NAC genes in Oryza sativa [7], NAC genes in Nicotiana tabacum [37], 163 NAC genes in Populus trichocarpa [38], 74 NAC genes in Vitis vinifera [39], 147 putative NAC genes in Setaria italic [40], 145 NAC genes in Gossypium raimondii [41], 167 NAC genes in Musa acuminate [42], 71 NAC genes in Cicer arietinum [43], 96 NAC genes in Manihot esculenta [44], 148 NAC genes in Zea mays [45], 79 NAC genes in Morus notabilis [46], 82 NAC genes in Cucumis melo [47], and 104 NAC genes in Solanum lycopersicum [48]
We identified 37 NAC genes from woodland strawberry and performed a detailed investigation of their phylogeny, conserved motifs, gene structure, expression profiles in various tissues and in response to cold, heat, drought, salt stress, signaling of H2O2, melatonin, abscisic acid (ABA) and rapamycin, and response to the pathogens Colletotrichum gloeosporioides and Ralstonia solanacearum

Summary

Introduction

Transcription factors containing the highly conserved NAC domain (NAM-ATAF1/2-CUC) in the N-terminal region compromise is one of the largest transcription factors families in plants [1,2,3]. The C-terminal region that contains the protein binding activity domain is greatly variable and plays an important role in transcriptional regulation [2, 4,5,6,7]. The NAC proteins play crucial roles in various aspects of plant growth and development No information is available on the NAC family in the rosaceous fruit crop, woodland strawberry (Fragaria vesca L.)

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