Genome-wide identification of MscS like gene family in Cicer arietinum using bioinformatics approach

Abstract

In plants, the mechanosensitive channels have important roles in proprioception, gravity sensing, growth of pollen tube and controlling the shape and size of plastid. In the present study, we performed computational analysis and identified the mechanosensitive channel of small conductance like (MscS) gene family in Cicer arietinum (chickpea). Six Cicer arietinum MscS-like (CaMSL) genes were identified. These six genes were distributed on four different chromosomes. A detailed overview of CaMSL genes in Cicer arietinum is explored by using a total of 33107 proteins from chickpea. On the basis of BLAST analysis, presence of complete ORF and evolutionary relationship we identified 6 genes (named as CaMSL1, CaMSL2, CaMSL3, CaMSL4, CaMSL5 and CaMSL6). Further, the transmembrane regions, subcellular localization, physico-chemical properties, gene structure analysis and genome structure annotation of the identified genes confirmed that the identified genes might act for transmembrane proteins as well as their subcellular localization prediction demonstrated three genes to be located in the plasma membrane, two in the chloroplast membrane, one in the mitochondrion. Thus, further analysis of the data obtained from this study will help to make a baseline to increase the understanding of involvement of MSL gene families in plant growth, development and other functions in C. arietinum.