Abstract
Ovarian tumor domain (OTU)-containing deubiquitinating enzymes (DUBs) are an essential DUB to maintain protein stability in plants and play important roles in plant growth development and stress response. However, there is little genome-wide identification and analysis of the OTU gene family in rice. In this study, we identified 20 genes of the OTU family in rice genome, which were classified into four groups based on the phylogenetic analysis. Their gene structures, conserved motifs and domains, chromosomal distribution, and cis elements in promoters were further studied. In addition, OTU gene expression patterns in response to plant hormone treatments, including SA, MeJA, NAA, BL, and ABA, were investigated by RT-qPCR analysis. The results showed that the expression profile of OsOTU genes exhibited plant hormone-specific expression. Expression levels of most of the rice OTU genes were significantly changed in response to rice stripe virus (RSV), rice black-streaked dwarf virus (RBSDV), Southern rice black-streaked dwarf virus (SRBSDV), and Rice stripe mosaic virus (RSMV). These results suggest that the rice OTU genes are involved in diverse hormone signaling pathways and in varied responses to virus infection, providing new insights for further functional study of OsOTU genes.
Highlights
By NCBI CD search and comparison with known rice Ovarian tumor domain (OTU) family proteins, we identified 20 non-redundant rice OTU genes, including 14 known rice OTU genes, and named them OsOTU1 to OsOTU20
We found that the expression levels of OsOTU18 and OsOTU20 were all upregulated to Rice stripe virus (RSV), rice black-streaked dwarf virus (RBSDV), Southern rice black-streaked dwarf virus (SRBSDV), and Rice stripe mosaic virus (RSMV) infection, while their expression levels were significantly downregulated after JA treatment, indicating that they may be susceptible to these four viruses
Our results showed that most rice OTU genes respond to viral infection
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Deubiquitination is a highly regulated process that is involved in many cellular functions, including cell cycle regulation [4], proteasome- and lysosome-dependent protein degradation [5,6], gene expression [7], DNA repair [8], kinase activation [9], and microbial pathogenicity [10]. Arabidopsis OTU DUBs are a kind of cysteine protease, which recognize and cleave three common types of ubiquitin linkages, including M1, K48, and K63 [17]. OTU plays an important role in plant immunity and pathogen invasion by regulating the ubiquitination of organisms and cleaving specific ubiquitin chains. In the arms race between pathogens and plants, pathogens gradually evolved a new mechanism They may synthesize cysteine protease with OTU domains themselves or interact with OTUs in plants to regulate ubiquitination and deubiquitination in plants, further affecting plant immune responses. We firstly investigated the genome-wide analysis of the rice OTU gene family and studied the expression pattern of OsOTU genes in response to different hormone treatments and various virus infection
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