Genome-Wide Identification and Gene Expression Analysis of ABA Receptor Family Genes in Brassica juncea var. tumida.

Cheng Cheng,Su Su,Li Li,Cai Cai,Zhong Zhong

doi:10.3390/genes10060470

Cheng Cheng, Su Su + Show 3 more

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https://doi.org/10.3390/genes10060470

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Journal: Genes	Publication Date: Jun 20, 2019
Citations: 9	License type: CC BY 4.0

Affiliation: Yangtze Normal University

Abstract

Abscisic acid (ABA) plays important roles in multiple physiological processes, such as plant response to stresses and plant development. The ABA receptors pyrabactin resistance (PYR)/ PYR1-like (PYL)/regulatory components of ABA receptor (RCAR) play a crucial role in ABA perception and signaling. However, little is known about the details regarding PYL family genes in Brassica juncea var. tumida. Here, 25 PYL family genes were identified in B. juncea var. tumida genome, including BjuPYL3, BjuPYL4s, BjuPYL5s, BjuPYL6s, BjuPYL7s, BjuPYL8s, BjuPYL10s, BjuPYL11s, and BjuPYL13. The results of phylogenic analysis and gene structure showed that the PYL family genes performed similar gene characteristics. By analyzing cis-elements in the promoters of those BjuPYLs, several hormone and stress related cis-elements were found. The results of gene expression analysis showed that the ABA receptor homologous genes were induced by abiotic and biotic stress. The tissue-specific gene expression patterns of BjuPYLs also suggested those genes might regulate the stem swelling during plant growth. These findings indicate that BjuPYLs are involved in plant response to stresses and organ development. This study provides valuable information for further functional investigations of PYL family genes in B. juncea var. tumida.

Highlights

The plant phytohormone abscisic acid (ABA) is involved in multiple plant physiological processes, such as seed dormancy, seed germination, seedling development, post-germinative growth, stomatal movement, and synthesis of storage proteins and lipids [1,2,3,4,5,6,7]
In Brassica napus, the transcript levels of BnPYLs genes were analyzed by qPCR in plant roots under multiple abiotic stresses treatment, and the results showed that the gene expression levels of BnPYR1-3, BnPYL1-2, and BnPYL7-2 were induced by drought, heat, and salinity treatments [16]
The results showed that BjuPYL4-1 and BjuPYL6-1 highly expressed in pod; BjuPYL4-1 and BjuPYL5-2 highly expressed in leaf; BjuPYL5-2, BjuPYL5-5, BjuPYL6-1, and BjuPYL7-3 highly expressed in swollen stem; BjuPYL5-2, BjuPYL6-1, and BjuPYL7-3 highly expressed in stem; BjuPYL4-1, BjuPYL5-2, and BjuPYL7-3 highly expressed in root, indicating that different BjuPYLs might be involved in different growth and development stages, and the expression patterns of BjuPYLs were existence of space-time specificity (Figure 5)

Summary

Introduction

The plant phytohormone abscisic acid (ABA) is involved in multiple plant physiological processes, such as seed dormancy, seed germination, seedling development, post-germinative growth, stomatal movement, and synthesis of storage proteins and lipids [1,2,3,4,5,6,7]. The core components of the ABA signaling pathway have been identified in Arabidopsis [9,10,11]. The ABA signaling pathway mainly contains pyrabactin resistance (PYR)/PYR1-like (PYL) regulatory components of ABA receptor (RCAR) protein family (ABA receptors), the co-receptors group A protein type 2C phosphatases (PP2Cs), and sucrose nonfermenting-1-related protein kinase class 2 (SnRK2s) [9,10,11]. In the absence of ABA, PP2Cs interact with SnRK2s and inhibit their kinase activity, which resulted in turning off of ABA signaling pathway. In the presence of ABA, PYR/PYL/RCAR receptors bind the hormone ABA, allowing them to physically associate with

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