Abstract
Nonspecific lipid transfer proteins (nsLTPs) play vital roles in lipid metabolism, cell apoptosis and biotic and abiotic stresses in plants. However, the distribution of nsLTPs in Arachis duranensis has not been fully characterized. In this study, we identified 64 nsLTP genes in A. duranensis (designated AdLTPs), which were classified into six subfamilies and randomly distributed along nine chromosomes. Tandem and segmental duplication events were detected in the evolution of AdLTPs. The Ks and ω values differed significantly between Types 1 and D subfamilies, and eight AdLTPs were under positive selection. The expression levels of AdLTPs were changed after salinity, PEG, low-temperature and ABA treatments. Three AdLTPs were associated with resistance to nematode infection, and DOF and WRI1 transcription factors may regulate the AdLTP response to nematode infection. Our results may provide valuable genomic information for the breeding of peanut cultivars that are resistant to biotic and abiotic stresses.
Highlights
Non-specific lipid transfer proteins are known to transfer various lipid molecules between lipid bilayers in plants
Based on both RNA-seq and quantitative real-time PCR (qRT-PCR) datasets, we found that AdLTPs are involved in responses to biotic and abiotic stresses
Identification and characterization of non-specific lipid transfer protein (nsLTP) genes (AdLTPs) in A. duranensis Using a bioinformatics approach, we identified a total of 64 AdLTP genes (Tables S1)
Summary
Non-specific lipid transfer proteins (nsLTPs) are known to transfer various lipid molecules between lipid bilayers in plants. NsLTP genes have different functions [3], including fertilization in flowering plants, adhesion of the pollen tube to the stigma, somatic embryogenesis, lipid metabolism, cutin synthesis, nitrogen fixation, fruit ripening, cell apoptosis, activation and regulation of various signaling cascades, and protection against and adaptation of plants to biotic and abiotic stresses [4,5,6,7,8]. Stress-resistant genes from wild-type species could be valuable resources for the improvement of the cultivated peanut. We used qRT-PCR to identify AdLTP genes that might contribute to abiotic stress, and we used RNA-seq data previously reported for nematode-infected plants [17] to identify AdLTP genes that potentially confer resistance to nematode infection. Our results provide a comprehensive analysis of AdLTP genes and provide preliminary information on specific AdLTP genes that may be involved in biotic and abiotic stress resistance
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