Abstract
Long non-coding RNAs (lncRNAs) are non-coding RNAs of more than 200 nucleotides. To date, the roles of lncRNAs in soybean fatty acid synthesis have not been fully studied. Here, the low-linolenic acid mutant ‘MT72′ and the wild-type control ‘JN18′ were used as materials. The lncRNAs in young pods at 30 and 40 days (d) after flowering were systematically identified and analyzed using transcriptome sequencing technology combined with bioinformatics tools. A total of 39,324 lncRNAs and 561 differentially expressed lncRNAs were identified. A lncRNAs-miRNAs-protein-coding genes (mRNAs) network was constructed, and 46 lncRNAs, 46 miRNAs and 137 mRNAs were found to be correlated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of 12 targeted mRNAs in the competing endogenous RNA network showed that these lncRNAs may be involved in the biological processes of fatty acid transport, lipid synthesis and cell division. Finally, the expression levels of differentially expressed lncRNAs, miRNAs and mRNAs were verified using qRT-PCR. The expression patterns of most genes were consistent with the sequencing results. In conclusion, new information was provided for the study of fatty acid synthesis by lncRNAs in young soybean pods.
Highlights
Soybean [Glycine max (L.) Merr] is an important oil crop that is planted w orldwide[1]
Bardou reported that Long non-coding RNAs (lncRNAs) Induced by Phosphate Star-Vation[1] (IPS1) induced by low phosphorus in A. thaliana removes the inhibition of miR399 on target gene PHO2 by simulating the target gene[18]
The results showed that some lncRNAs may have fatty acid transport (GO:0,015,908), lipid metabolic process (GO:0,006,629) and other functions related to oil synthesis
Summary
Soybean [Glycine max (L.) Merr] is an important oil crop that is planted w orldwide[1]. Recent studies have found that lncRNAs are key regulators of cellular processes and can play roles at the transcription level as cis- or trans-regulators of gene expression with functions in regulating plant growth and d evelopment[6]. Chen identified 3,030 long intergenic non-coding RNAs (lincRNAs) and 275 natural antisense transcripts (lncNATs) in soybean roots by high-throughput s equencing[14] These lncRNAs induced by continuous salt-stress and their potential functions in soybean roots were explored. The expression profiles of lncRNAs at different stages in the young pods of the low-linolenic acid mutant ‘MT72′ and wild-type ‘JN18′ were systematically identified and analyzed. We compared the levels of differentially expressed lncRNAs with those of protein-coding genes, and we inferred that some lncRNAs had potential functions related to the regulation of soybean lipid anabolism using a ceRNA co-expression network. This research provides insights into the regulation of ceRNAs and other biological processes in soybean, and it provides theoretical basis into the functions of lncRNAs in soybean and other oil-bearing crops
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
