Abstract
Tartary buckwheat (TB; Fagopyrum tataricum Gaertn.) is an important multigrain crop and medicinal plant, but functional genomics and molecular breeding research in this species have been lacking for quite some time. Here, genome-wide screening was performed to develop simple sequence repeat (SSR) markers associated with six major agronomic traits and the rutin contents of 97 core germplasm resources. A total of 40,901 SSR loci were identified; they were uniformly distributed throughout the TB genome, with a mean distance of 11 kb between loci. Based on these loci, 8,089 pairs of SSR primers were designed, and 101 primer pairs for polymorphic SSR loci were used to genotype the 97 core germplasm resources. The polymorphic SSR loci showed high genetic variation in these core germplasm resources, with an average polymorphic information content (PIC) value of 0.48. In addition, multiple SSR markers, such as SXAU8002 [100-grain weight (HGW)] and SXAU8006 [stem diameter (SD)], were found to be associated with agronomic traits in the two environments. Finally, based on gene functional annotation and homology analysis, a candidate gene, FtPinG0007685500, that may affect the node number and SD of the main stem by participating in lignin synthesis was identified. This study reports the mining of genome-wide SSR loci and the development of markers in TB, which can be used for molecular characterization of the germplasm in its gene pool. In addition, the detected markers and candidate genes could be used for marker-assisted breeding and functional gene cloning in TB.
Highlights
Tartary buckwheat (TB; Fagopyrum tataricum Gaertn.) is a species of Fagopyrum that is recognized as an important edible crop and medicinal plant due to its high abundance of flavonoids with antioxidant activity (Zhang et al, 2012b; Hou et al, 2021)
lateral branches (LBs), root length (RL), and stem diameter (SD) showed relatively large variation coefficients, indicating that the materials selected for this study were broadly representative of these traits
Agronomic traits are the unique traits of crop varieties that are determined by their genetic background and the influence of the environment (Cao et al, 2016)
Summary
Tartary buckwheat (TB; Fagopyrum tataricum Gaertn.) is a species of Fagopyrum that is recognized as an important edible crop and medicinal plant due to its high abundance of flavonoids with antioxidant activity (Zhang et al, 2012b; Hou et al, 2021). Rutin (quercetin3-O-rutinoside) is one of many well-known flavonoids with high biological activity. This compound exerts antioxidant and antiinflammatory effects by inhibiting blood platelet aggregation, increasing capillary strength, and reducing cholesterol levels in the blood (Luthar et al, 2020). As TB is a typical functional food resource, it is necessary to analyze the genetic basis of its important agronomic and quality traits, such as plant height (PH), yield, and rutin content
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