Abstract
The HD-Zip IV transcription factors, which belong to the homeodomain leucine zipper (HD-ZIP) family, are essential for the development of epidermal cells in plants. However, there is limited information about HD-Zip IV in tea plant (Camellia sinensis L.). Here we identified 10 HD-Zip IV members (CsHDZIV1∼10) in tea plant, which are distributed in 8 chromosomes with two segmentally duplicated gene pairs. The exon number of CsHDZIVs ranges from 9 to 11, with most genes having two conserved motifs in the 3’UTR. CsHDZIV1∼9 have almost identical motifs and tertiary structures. The promoter region of CsHDZIVs was predicted to harbor light, phytohormone, stress, growth and developmental elements. 9 CsHDZIVs are divided into 5 subfamilies and located in the nucleus. They exhibited expression trends across 12 tea plant tissues and responded to PEG, cold, NaCl and shading treatments. CsHDZIV3 overexpression partially and completely restored trichome development defects of gl2 leaves and stems, respectively. CsHDZIV3 showed histochemical staining in Arabidopsis trichomes and protein interaction with itself. It might regulate trichome development through forming homodimers. These findings contribute to functional study of tea plant HD-Zip IV and provide guiding significance for the investigation of trichome development and the improvement of trichome traits.
Published Version
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