Genome-wide analysis of MATE transporters and response to metal stress in Cajanus cajan

Biying Dong,Lili Niu,Dong Meng,Zhihua Song,Litao Wang,Yue Jian,Xiaohong Fan,Mingzhu Dong,Qing Yang,Yujie Fu

doi:10.1080/17429145.2019.1620884

Abstract

ABSTRACT Multidrug and Toxic Compound Extrusion (MATEs) is one of the characteristic transporter families, which plays a key role in the detoxication of endogenous secondary metabolites and exogenous agents in both animal and plant cells. In this study, we identified a total of 67 MATE genes (CcMATEs) from the pigeonpea genome, on which we performed bioinformatics analysis and we group them by phylogenetic analysis. Finally, eight represented CcMATE genes were selected for further qRT-PCR analysis of tissue specificity and response to metal stress in pigeonpea. The results showed that both CcMATE34 and 45 genes were significantly up-regulated and the CcMATE4 gene was only up-regulated in the roots under the stress of Al, Mn and Zn. We speculated that the function of CcMATE34 and 45 might be related to the transport of alkaloids and harmful substances and the function of CcMATE4 might be related to the delivery of flavonoids.

Highlights

multidrug and toxin extrusion (MATE) (Multidrug and Toxic Compound Extrusion) transporters are secondary transporters of highly conserved cations and are widely present in archaea, bacteria and eukaryotes (Brown et al 1999)
AtALF5 (Arabidopsis thaliana aerrant lateral root formation 5) is the first MATE protein identified in plants (Diener et al 2001), after which the first multi-specific MATE transporter AtDTX1 is identified in Arabidopsis thaliana in 2002 (Li et al 2002)
A BLASTP search was performed on the NCBI website based on the 57 MATE protein sequences in Arabidopsis thaliana

Summary

Introduction

MATE (Multidrug and Toxic Compound Extrusion) transporters are secondary transporters of highly conserved cations and are widely present in archaea, bacteria and eukaryotes (Brown et al 1999). Most members of this family have 450– 550 amino acid residues; a few members can reach 700 with 9–12 transmembrane helices in the sequence (Borges-Walmsley et al 2003). The functions of the MATE protein family are diverse It involved plants’ numerous growth and development function. The other functions are participating in root detoxification by transporting heterologous harmful substances, transporting salicylic acid, alkaloids, antibiotics and other toxic compounds, maintaining the balance of iron ions in plants, maintaining plants by regulating the development of lateral organs, enhancing the aluminum tolerance of plants by enhancing the secretion of citric acid, directly or indirectly involving in the detoxification of toxic compounds or metals, etc. (Nesi et al 2001; Li, He, et al 2002; Rogers and Guerinot 2002; Furukawa et al 2007; Zhou et al 2013; Li et al 2014; Wu et al 2014)

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