Abstract
Gymnadenia conopsea (Fragrant orchid) is a widespread model species for the study of polymorphism in the European part of Russia and abroad. Flow cytometry refers to rapid methods effective for assessing the structure of a population by DNA size estimation. However, this method is only available in habitats with close proximity to the place of the experiment, because this indicator is determined only in fresh leaves. A small population of G. conopsea in the industrial area adjacent to the Novosibirsk urban agglomeration in the Novosibirsk Region (NR) was studied. Only diploids were detected in the G. conopsea cenopopulation occurred in the industrial zone of the NR. In this study we pointed on significance of genome size variation, which correlates with the adaptive evolution of the G. conopsea in West Siberia. The revealed absence of minor cytotypes (individuals with a higher level of ploidy) in G. conopsea population reduces intraspecific and intrapopulation diversity of the species.
Highlights
G. conopsea constitutes a significant part of the orchid flora from the temperate zone and becomes a model species for studying the effect of ploidy changes on the plant organism [3]
While under undisturbed conditions in the northern regions of Russia, G. conopsea populations with different cytotypes are existing, we revealed the lack of genome size variability among studied individuals in the cenopopulation occurred in the industrial zone of the Novosibirsk Region (NR)
Due to the complex genome analysis in the studied CP from Novosibirsk region we revealed only diploid cytotypes within G. conopsea samples
Summary
Br. is a polycarpous tuberous species with erect aerial shoots, which is widespread throughout Eurasia [1, 2]. G. conopsea constitutes a significant part of the orchid flora from the temperate zone and becomes a model species for studying the effect of ploidy changes on the plant organism [3]. Genome size of the orchid species has not been investigated in Asiatic part of Russia in natural environments. Investigators successfully applied genome size data in determination of levels of genetic diversity of threatened plant species [4] and identification of species [5]. Studies relating plant genome size variation to adverse environmental conditions are scarce [6]
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