Abstract

Bradyrhizobium sp. strain WSM471 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen- (N2) fixing root nodule formed on the annual legume Ornithopus pinnatus (Miller) Druce growing at Oyster Harbour, Albany district, Western Australia in 1982. This strain is in commercial production as an inoculant for Lupinus and Ornithopus. Here we describe the features of Bradyrhizobium sp. strain WSM471, together with genome sequence information and annotation. The 7,784,016 bp high-quality-draft genome is arranged in 1 scaffold of 2 contigs, contains 7,372 protein-coding genes and 58 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program.

Highlights

  • The most abundant form of nitrogen (N) occurs in the atmosphere as a chemically inert dinitrogen (N2) gas

  • The highest contribution to biological fixation occurs from the process of symbiotic nitrogen fixation (SNF)

  • Because of its superior capacity to fix nitrogen with O. sativus relative to other strains of Bradyrhizobium, strain WSM471 was released as a commercial inoculant for this legume in Australia in 1996 [7] and remains in current usage

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Summary

Introduction

The most abundant form of nitrogen (N) occurs in the atmosphere as a chemically inert dinitrogen (N2) gas. To extend productive crops and pastures into these regions, considerable efforts have been devoted to sourcing legume hosts and their compatible microsymbionts from different geographical locations that are edaphically and climatically suited to the challenging areas into which they are to be introduced [3]. Because of its superior capacity to fix nitrogen with O. sativus relative to other strains of Bradyrhizobium, strain WSM471 was released as a commercial inoculant for this legume in Australia in 1996 [7] and remains in current usage. This strain is the commercial “back-up” for inoculation of lupins in Australia. We present a summary classification and a set of general features for Bradyrhizobium sp. strain WSM471 together with the description of the complete genome sequence and its annotation

Classification and general features
Not recorded
Genome sequencing and annotation information
Genome annotation
Genome properties
CRISPR repeats
Findings
Acknowledg ements
Full Text
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