Abstract
Dehalogenimonas alkenigignens IP3-3T is a strictly anaerobic, mesophilic, Gram negative staining bacterium that grows by organohalide respiration, coupling the oxidation of H2 to the reductive dehalogenation of polychlorinated alkanes. Growth has not been observed with any non-polyhalogenated alkane electron acceptors. Here we describe the features of strain IP3-3T together with genome sequence information and its annotation. The 1,849,792 bp high-quality-draft genome contains 1936 predicted protein coding genes, 47 tRNA genes, a single large subunit rRNA (23S-5S) locus, and a single, orphan, small unit rRNA (16S) locus. The genome contains 29 predicted reductive dehalogenase genes, a large majority of which lack cognate genes encoding membrane anchoring proteins.
Highlights
Strain IP3-3T (=JCM 17062, =NRRL B-59545) is the type strain of the species Dehalogenimonas alkenigignens [1]
Construction of 16S rRNA gene libraries indicated that bacteria closely related or identical to D. alkenigignens were present at high relative abundance in the groundwater where strains IP3-3T and SBP-1 were first isolated [1]
Strains of D. alkenigignens possess the unique trait of growing via organohalide respiration, a process in which halogenated organic compounds are utilized as terminal electron acceptors
Summary
Strain IP3-3T (=JCM 17062, =NRRL B-59545) is the type strain of the species Dehalogenimonas alkenigignens [1]. Based on 16S rRNA gene sequences, the closest related type strains are Dehalogenimonas lykanthroporepellens BL-DC-9T [1, 5] and Dehalococcoides mccartyi 195T [6], with sequence identities of 96.2 and 90.6 %, respectively [1]. Growth conditions and genomic DNA preparation D. alkenigignens strain IP3-3T (=JCM 17062, =NRRL B59545) was cultured in liquid anaerobic basal medium [1] supplemented with 2 mM 1,2-dichloropropane. The genomes of D. alkenigignens IP3-3T, D. lykanthroporepellens BL-DC-9T [21], and Dehalococcoides mccartyi strains [22,23,24] contain similar number of rRNA and tRNA encoding genes, they lack overall synteny and differ in their GC content, gene density, and percentage of sequence that encodes proteins.
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